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linear hygromycin marker takara bio  (TaKaRa)


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    Structured Review

    TaKaRa linear hygromycin marker takara bio
    Linear Hygromycin Marker Takara Bio, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/linear+hygromycin+marker/Linear+Hygromycin+Marker/pm42034057-258-35-38
    Average 93 stars, based on 111 article reviews
    linear hygromycin marker takara bio - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: New benzoyl sesquiterpenoid, aoganolide, produced by Talaromyces sp. KTF-0021 strain (laeA-introduced mutant of FKI-5759 strain).
    Article Snippet: To discover novel natural compounds by awakening unexplored or silent BGCs in fungi, our research group has explored the cultured broths of the laeA-introduced mutant fungal strains.. In this study, we report the isolation of a new benzoyl sesquiterpenoid, aoganolide (4), as well as three known compounds, decarboxyaltenusin (1), altenusin (2), and penipyranicin B (3), from the cultured broth of Talaromyces sp. KTF-0021, which was a laeA-introduced mutant of FKI-5759 strain.. The planar structure of 4 was elucidated by spectroscopic analysis and the absolute configuration of 4 was determined by the calculated ECD spectral method.

    Marker:

    Article Title: New benzoyl sesquiterpenoid, aoganolide, produced by Talaromyces sp. KTF-0021 strain (laeA-introduced mutant of FKI-5759 strain).
    Article Snippet: To discover novel natural compounds by awakening unexplored or silent BGCs in fungi, our research group has explored the cultured broths of the laeA-introduced mutant fungal strains.. In this study, we report the isolation of a new benzoyl sesquiterpenoid, aoganolide (4), as well as three known compounds, decarboxyaltenusin (1), altenusin (2), and penipyranicin B (3), from the cultured broth of Talaromyces sp. KTF-0021, which was a laeA-introduced mutant of FKI-5759 strain.. The planar structure of 4 was elucidated by spectroscopic analysis and the absolute configuration of 4 was determined by the calculated ECD spectral method.

    Article Title: Achaete‐scute family bHLH transcription factor 2 activation promotes hepatoblastoma progression
    Article Snippet: .. To generate a double‐stable clone capable of high ASCL2 induction levels, the pTRE ASCL2 was co‐transfected into HepG2 Tet‐On 3G along with a linear hygromycin marker (Hyg r ; Clontech), and colonies were selected with 600 μg/mL hygromycin. ..

    Article Title: Structural Characterization of 5-Substituted Pyrrolo[3,2- d ]pyrimidine Antifolate Inhibitors in Complex with Human Serine Hydroxymethyl Transferase 2.
    Article Snippet: We previously discovered first-in-class multitargeted 5-substituted pyrrolo[3,2-d]pyrimidine antifolates that inhibit serine hydroxymethyltransferase 2 (SHMT2), resulting in potent in vitro and in vivo antitumor efficacies.. In this report, we present crystallographic structures for SHMT2 in complex with an expanded series of pyrrolo[3,2-d]pyrimidine compounds with variations in bridge length (3−5 carbons) and the side chain aromatic ring (phenyl, thiophene, fluorine-substituted phenyl, and thiophene).. We evaluated structural features of the inhibitorSHMT2 complexes and correlations to inhibitor potencies (i.e., Kis), highlighting conserved polar contacts and identifying 5carbon bridge lengths as key determinants of inhibitor potency.

    Article Title: ZC3H11A interacts with PABPN1 and alters polyadenylation of viral transcripts
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Article Title: The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Article Title: Achaete-scute family bHLH transcription factor 2 activation promotes hepatoblastoma progression.
    Article Snippet: .. To generate a double- stable clone capable of high ASCL2 induction levels, the pTRE ASCL2 was co- transfected into HepG2 Tet- On 3G along with a linear hygromycin marker (Hygr; Clontech), and colonies were selected with 600 μg/mL hygromycin. ..

    Article Title: Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations.
    Article Snippet: .. U2OS-PBmal1::Fluc/ΔBmal1 cells were transfected with 5 μg pTetOne-MycBmal1 plasmid and 0.25 μg linear hygromycin marker (Takara Bio USA, San Jose, CA, USA) using Xfect transfection reagent (Takara Bio USA, San Jose, CA, USA). ..

    Article Title: The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts.
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Knock-Out:

    Article Title: Structural Characterization of 5-Substituted Pyrrolo[3,2- d ]pyrimidine Antifolate Inhibitors in Complex with Human Serine Hydroxymethyl Transferase 2.
    Article Snippet: We previously discovered first-in-class multitargeted 5-substituted pyrrolo[3,2-d]pyrimidine antifolates that inhibit serine hydroxymethyltransferase 2 (SHMT2), resulting in potent in vitro and in vivo antitumor efficacies.. In this report, we present crystallographic structures for SHMT2 in complex with an expanded series of pyrrolo[3,2-d]pyrimidine compounds with variations in bridge length (3−5 carbons) and the side chain aromatic ring (phenyl, thiophene, fluorine-substituted phenyl, and thiophene).. We evaluated structural features of the inhibitorSHMT2 complexes and correlations to inhibitor potencies (i.e., Kis), highlighting conserved polar contacts and identifying 5carbon bridge lengths as key determinants of inhibitor potency.

    Transfection:

    Article Title: Structural Characterization of 5-Substituted Pyrrolo[3,2- d ]pyrimidine Antifolate Inhibitors in Complex with Human Serine Hydroxymethyl Transferase 2.
    Article Snippet: We previously discovered first-in-class multitargeted 5-substituted pyrrolo[3,2-d]pyrimidine antifolates that inhibit serine hydroxymethyltransferase 2 (SHMT2), resulting in potent in vitro and in vivo antitumor efficacies.. In this report, we present crystallographic structures for SHMT2 in complex with an expanded series of pyrrolo[3,2-d]pyrimidine compounds with variations in bridge length (3−5 carbons) and the side chain aromatic ring (phenyl, thiophene, fluorine-substituted phenyl, and thiophene).. We evaluated structural features of the inhibitorSHMT2 complexes and correlations to inhibitor potencies (i.e., Kis), highlighting conserved polar contacts and identifying 5carbon bridge lengths as key determinants of inhibitor potency.

    Article Title: Achaete-scute family bHLH transcription factor 2 activation promotes hepatoblastoma progression.
    Article Snippet: .. To generate a double- stable clone capable of high ASCL2 induction levels, the pTRE ASCL2 was co- transfected into HepG2 Tet- On 3G along with a linear hygromycin marker (Hygr; Clontech), and colonies were selected with 600 μg/mL hygromycin. ..

    Article Title: Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations.
    Article Snippet: .. U2OS-PBmal1::Fluc/ΔBmal1 cells were transfected with 5 μg pTetOne-MycBmal1 plasmid and 0.25 μg linear hygromycin marker (Takara Bio USA, San Jose, CA, USA) using Xfect transfection reagent (Takara Bio USA, San Jose, CA, USA). ..

    Mutagenesis:

    Article Title: Structural Characterization of 5-Substituted Pyrrolo[3,2- d ]pyrimidine Antifolate Inhibitors in Complex with Human Serine Hydroxymethyl Transferase 2.
    Article Snippet: We previously discovered first-in-class multitargeted 5-substituted pyrrolo[3,2-d]pyrimidine antifolates that inhibit serine hydroxymethyltransferase 2 (SHMT2), resulting in potent in vitro and in vivo antitumor efficacies.. In this report, we present crystallographic structures for SHMT2 in complex with an expanded series of pyrrolo[3,2-d]pyrimidine compounds with variations in bridge length (3−5 carbons) and the side chain aromatic ring (phenyl, thiophene, fluorine-substituted phenyl, and thiophene).. We evaluated structural features of the inhibitorSHMT2 complexes and correlations to inhibitor potencies (i.e., Kis), highlighting conserved polar contacts and identifying 5carbon bridge lengths as key determinants of inhibitor potency.

    Construct:

    Article Title: Structural Characterization of 5-Substituted Pyrrolo[3,2- d ]pyrimidine Antifolate Inhibitors in Complex with Human Serine Hydroxymethyl Transferase 2.
    Article Snippet: We previously discovered first-in-class multitargeted 5-substituted pyrrolo[3,2-d]pyrimidine antifolates that inhibit serine hydroxymethyltransferase 2 (SHMT2), resulting in potent in vitro and in vivo antitumor efficacies.. In this report, we present crystallographic structures for SHMT2 in complex with an expanded series of pyrrolo[3,2-d]pyrimidine compounds with variations in bridge length (3−5 carbons) and the side chain aromatic ring (phenyl, thiophene, fluorine-substituted phenyl, and thiophene).. We evaluated structural features of the inhibitorSHMT2 complexes and correlations to inhibitor potencies (i.e., Kis), highlighting conserved polar contacts and identifying 5carbon bridge lengths as key determinants of inhibitor potency.

    Sequencing:

    Article Title: ZC3H11A interacts with PABPN1 and alters polyadenylation of viral transcripts
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Article Title: The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Article Title: The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts.
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Plasmid Preparation:

    Article Title: ZC3H11A interacts with PABPN1 and alters polyadenylation of viral transcripts
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Article Title: The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Article Title: Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations.
    Article Snippet: .. U2OS-PBmal1::Fluc/ΔBmal1 cells were transfected with 5 μg pTetOne-MycBmal1 plasmid and 0.25 μg linear hygromycin marker (Takara Bio USA, San Jose, CA, USA) using Xfect transfection reagent (Takara Bio USA, San Jose, CA, USA). ..

    Article Title: The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts.
    Article Snippet: .. Briefly, a DNA fragment containing the U6 promoter, the gRNA sequence, the gRNA scaffold sequence, and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). ..

    Stable Transfection:

    Article Title: Achaete-scute family bHLH transcription factor 2 activation promotes hepatoblastoma progression.
    Article Snippet: .. To generate a double- stable clone capable of high ASCL2 induction levels, the pTRE ASCL2 was co- transfected into HepG2 Tet- On 3G along with a linear hygromycin marker (Hygr; Clontech), and colonies were selected with 600 μg/mL hygromycin. ..



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